Protocols

Choose from the following categories of lab protocols. You can also access these pages from the drop-down menu above. Unpublished protocols are password protected.

1. Ajo-Franklin Lab Manual
2. Equipment SOPs
3. General molecular biology
4. General cell culture
5. Microbial electrochemistry
6. Anaerobic iron respiration
7. Carbonate mineralization
8. Cytochrome c expression & characterization
   
   1. Overexpression of c-Cytochromes in organisms besides E. coli
   2. Screening strains for cyt c expression in 96-well plates
   3. Cyt c expression: Aerobic expression in flasks
   4. Detecting CcmE via immunoblotting
   5. ECL analysis of cyt c expressing strains
   6. Detecting cyt c using TMBZ (heme) staining of PAGE gels
   7. Measuring Cyt c content of intact bacterial cells using diffuse reflectance
9. Non-bio synthesis
10. S-layers
11. Supported membranes
    1. Biotage SOP
12. Optical imaging
13. SEM imaging
14. Recipes
    1. Antibiotics
    2. LB Media & Plates
15. Equipment SOPs
    1. Biotage
    2. Anaerobic Chamber
    3. FACS Aria