The Basics

1. Agarose Gel Electrophoresis to Analyze DNA Fragments
2. PCR primer design
3. PCR amplification from gDNA or plasmid DNA
4. Preparative Scale Restriction Digests of PCR products and Plasmids
5. Ligation of Plasmid and Inserts
6. Transformation of Chemically Competent Bacterial Cells
7. Analytical Scale Restriction Digests of PCR products and Plasmids
8. Commonly Used Sequencing Primers
9. Plasmid Verification
10. Archiving a Strain and/or Plasmid

Specialized Cloning

1. Making ds DNA inserts from Oligonucleotides
2. Site-Directed Mutagenesis of Plasmids
3. Phusion Based Modification of Plasmids for Golden Gate Cloning
4. Golden Gate Cloning
5. Preparation of Electrocompetent Cells
6. Transformation of Electrocompetent Bacterial Cells using Electroporation
7. Doing 48 minipreps in parallel using the Qiagen Turbo Kit
8. Genomic Deletions and Insertions with Lambda Red
9. Single Colony PCR