- Heat a 20% (v/v) solution of 7x detergent (www.mpbio.com) on a hot plate until solution is clear, typically solution is 90 °C. WARNING: Do not leave unattended!
- Place coverslips (25×25, #1) in a ceramic holder
- Immerse coverslips (25×25, #1) in a ceramic holder into solution for 5 minutes
- Rinse extensively with ddH2O.
- Dry cover slips with N2 gas. Place in container and cover with foil.
- Plasma-etch coverslips in UV-ozone chamber (located on 4th floor) for 5 minutes.