To make M1 medium:
Add the following to the 1L ddH2O, adjust to pH=7.4 and autoclave.
| Component | Conc (mM) | MW (g/mol) | add per 1L (g) |
| PIPES | 50 | 302.37 | 15.12 |
| NaOH | 7.5 | 40 | 0.30 |
| NH4Cl | 28 | 53.49 | 1.5 |
| KCl | 1.3 | 74.55 | 0.10 |
| NaH2PO4•H2O | 4.3 | 137.99 | 0.59 |
| NaCl | 100 | 58.44 | 5.84 |
| After autoclaving: | |||
| 100X vitamin stock | — | — | 10 mL |
| 100X mineral stock | — | — | 10 mL |
| 100 X amino acid stock | — | — | 10 mL |
| Optional: | |||
| Casamino acids | 0.2% | 2 g | |
| sodium lactate | As needed |
100X vitamin stock:
Add the following to 1 L ddH2O, filter sterilize. Store at 4°C, protect from light.
| vitamin | add to 1 L (mg) |
| D-biotin (B7) | 2 |
| folic acid (B9) | 2 |
| pyridoxine HCl (B6) | 10 |
| thiamine HCl (B1) | 5 |
| nicotinic acid (B3) | 5 |
| D-pantothenic acid, hemicalcium salt (B5) | 5 |
| cobalamin (B12) | 0.1 |
| p-aminobenzoic acid | 5 |
| a-lipoic acid | 5 |
100X amino acid stock:
Add the following to 1 L ddH2O, adjust to pH=7.0. Filter sterilize and store at 4°C.
| amino acid | add to 1 L (g) |
| L-glutamic acid | 2 |
| L-arginine | 2 |
| D,L-serine | 2 |
100X trace mineral stock:
Add the following to 1 L ddH2O, adjust to pH=7.0. Filter sterilize and store at 4°C.
| mineral | conc. (mM) | MW (g/mol) | add per 1 L (mg) |
| C6H9NO6Na3 (Na-NTA) | 7.85 | 257 | 2017.4 |
| MgSO4·7H2O | 12.17 | 256.47 | 3121.2 |
| MnSO4·H2O | 2.96 | 169.02 | 500.3 |
| NaCl | 17.11 | 58.44 | 1000.0 |
| FeSO4·7H2O | 0.36 | 278.05 | 100.0 |
| CaCl2·2H2O | 0.68 | 147.014 | 100.0 |
| CoCl2·6H2O | 0.42 | 237.93 | 100.0 |
| ZnCl2 | 0.95 | 136.315 | 129.5 |
| CuSO4·5H2O | 0.040 | 249.7 | 10.0 |
| AlK(SO4)2·12H2O | 0.021 | 474.39 | 10.0 |
| H3BO3 | 0.016 | 61.83 | 1.0 |
| Na2MoO4·2H2O | 0.010 | 241.95 | 2.4 |
| NiCl2·6H2O | 0.010 | 237.69 | 2.4 |
| Na2WO4·2H2O | 0.076 | 329.85 | 25.1 |